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探讨海藻酸钠溶液的浓度变化对藻酸盐水凝胶新型组织工程支架结构与性能的影响.在氯化钙溶液浓度相同的条件下,改变海藻酸钠溶液的浓度,制备出不同原料配比的藻酸盐水凝胶,冷冻干燥后于光学显微镜下观察其微观结构变化,测定水凝胶的含水率与凝胶质量分数;将无菌的海藻酸钠溶液及间充质干细胞混合后,与氯化钙溶液反应得到包载干细胞的藻酸盐水凝胶,倒置荧光显微镜下观察水凝胶所释放出细胞的存活情况.在氯化钙浓度一定的条件下,随着海藻酸钠浓度的减小,水凝胶的空间网络结构由致密变得疏松,更易于细胞的生存与释放.实验表明,海藻酸钠水凝胶作为组织工程支架,用于神经损伤后修复具有较好的应用前景.
Abstract：To investigate the influence of the different sodium alginate concentration on the structure and property of hydrogel as scaffold for tissue engineering. With the same concentration of calcium chloride solution,different concentration of sodium alginate solution was mixed to prepare different types of hydrogels. The water content of hydrogel and gel mass fraction were determined,and their freeze dried microstructure changes were observed under the optical microscope. To obtain the hydrogel loading mesenchymal stem cells,the sterile sodium alginate solution and stem cells were mixed and reacted with calcium chloride solution. Then the survival cells released from the different hydrogels were observed using an inverted fluorescence microscopeation. Under the condition of certain concentration of calcium chloride,the network structure of hydrogel was becoming looser and easier for stem cells to survive and release,with the decrease of the concentration of sodium alginate. It could be concluded that sodium alginate hydrogel as tissue engineering scaffold had a good prospect for nerve injury repair application.
[1]Brian J C,Nobuko U,Stanley J T,et al.Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(39):14069-14074.
[2]Andrea J M,Roger Y T,Tasneem Z,et al.Repair of the injured spinal cord by transplantation of neural stem cells in a hyaluronan-based hydrogel[J].Biomaterials,2013,34(15):3775-3783.
[3]Sudhakar C K,Nitish U,Ankush J,et al.Nanotechnology Applications for Tissue Engineering[M].Atlanta:Elsevier,2015,77-94.
[4]王海宝,马学强.雪旺细胞-海藻酸钙凝胶移植对大鼠脊髓损伤后神经丝蛋白表达的影响[J].中医正骨,2008,20(6):1-3.
[5]Keren Z,Harald N,Yael B H,et al.A tunable silk-alginate hydrogel scaffold for stem cell culture and transplantation[J].Biomaterials,2014,35(12):3736-3743.
[6]Eduardo A,Pauline D B,Bernard U,et al.Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury[J].International Journal of Pharmaceutics,2013,455(1/2):148-158.
[7]Jinah J,Young J S,Hyeon J K,et al.Effects of alginate hydrogel cross-linking density on mechanical and biological behaviors for tissue engineering[J].Journal of the Mechanical Behavior of Biomedical Materials,2014,37:69-77.
[8]余晨晨.明胶/海藻酸盐复合凝胶微球支架的构建与表征[D].广州:华南理工大学,2014.
[9]Cristiana B da C,Darinka D K,Li W W A,et al.Influence of the stiffness of three-dimensionalalginate/collagen-I interpenetrating networks on fibroblast biology[J].Biomaterials,2014,35(32):8927-8936.
[10]Shoichet M S,Li R H,White M L,et al.Stability of hydrogelsused in cell encapsulation:an in vitro comparison of alginate andagarose[J].Biotechnology and Bioengineering,1996,50:374-381.
基本信息:
中图分类号:R318.08
引用信息:
[1]张琨,马珊珊,孟楠,等.海藻酸钠浓度对新型神经组织工程支架结构与性能的影响[J],2015,47(04):99-102.
基金信息:
河南省博士后科研项目,编号2014020